Protein-protein complexation in bioluminescence

Humic acid protein complexation

Interactions of purified Aldrich humic acid (PAHA) with lysozyme (LSZ) are investigated. In solution LSZ is moderately positively and PAHA negatively charged at the investigated pH values. The proton binding of PAHA and of LSZ is determined by potentiometric proton titrations at various KCl concentrations. It is also measured for two mixtures of PAHA–LSZ and compared with theoretically calculat...

Identification of protein-protein complexation area

The subject of the paper is to find an analytical formula describing a relationship between themaximum value of an annual tree stand height increment and the age at which it takes place. The 19height growth benchmarks representing all site quality indexes of two species: beech and spruce weretaken into consideration.

Protein-protein interactions of mitochondrial-associated protein via bioluminescence resonance energy transfer

Protein-protein interactions are essential biological reactions occurring at inter- and intra-cellular levels. The analysis of their mechanism is generally required in order link to understand their various cellular functions. Bioluminescence resonance energy transfer (BRET), which is based on an enzymatic activity of luciferase, is a useful tool for investigating protein-protein interactions i...

A Simple Complexation Model and the Experimental Datafor Protein Extraction Using Reverse Micellar Systems

In Vivo Analysis of Protein–Protein Interactions with Bioluminescence Resonance Energy Transfer (BRET): Progress and Prospects

Proteins are the elementary machinery of life, and their functions are carried out mostly by molecular interactions. Among those interactions, protein-protein interactions (PPIs) are the most important as they participate in or mediate all essential biological processes. However, many common methods for PPI investigations are slightly unreliable and suffer from various limitations, especially i...